کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1316178 | 976433 | 2011 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Tungsten-dependent formaldehyde ferredoxin oxidoreductase: Reaction mechanism from quantum chemical calculations Tungsten-dependent formaldehyde ferredoxin oxidoreductase: Reaction mechanism from quantum chemical calculations](/preview/png/1316178.png)
Formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus is a tungsten-dependent enzyme that catalyzes the oxidation of formaldehyde to formic acid. In the present study, quantum chemical calculations are used to elucidate the reaction mechanism of this enzyme. Several possible mechanistic scenarios are investigated with a large model of the active site designed on the basis of the X-ray crystal structure of the native enzyme. Based on the calculations, we propose a new mechanism in which the formaldehyde substrate binds directly to the tungsten ion. WVI=O then performs a nucleophilic attack on the formaldehyde carbon to form a tetrahedral intermediate. In the second step, which is calculated to be rate limiting, a proton is transferred to the second-shell Glu308 residue, coupled with a two-electron reduction of the tungsten ion. The calculated barriers for the mechanism are energetically very feasible and in relatively good agreement with experimental rate constants. Three other second-shell mechanisms, including one previously proposed based on experimental findings, are considered but ruled out because of their high barriers.
Quantum chemical calculations of the active site suggest a first-shell mechanism for the tungsten-dependent formaldehyde ferredoxin oxidoreductase. W=O performs a nucleophilic attack on the formaldehyde followed by a proton transfer to the second-shell Glu308 residue, which is coupled with a two-electron reduction of the tungsten ion.Figure optionsDownload as PowerPoint slide
Journal: Journal of Inorganic Biochemistry - Volume 105, Issue 7, July 2011, Pages 927–936