کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1316868 976489 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mechanistic aspects of the Fenton reaction under conditions approximated to the extracellular fluid
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Mechanistic aspects of the Fenton reaction under conditions approximated to the extracellular fluid
چکیده انگلیسی

The Fenton reaction was investigated, in a medium approximating to that of the extracellular fluid (ECF), by rapid-mixing stopped flow experiments and HPLC analysis using sodium terephthalate (TA2−). The reactive intermediate of the Fenton reaction hydroxylates the essentially nonfluorescent, TA2− to the brilliant fluorophor 2-hydroxy-terephthalate (OH-TA), which allows the Fenton reaction to be monitored in stopped-flow experiments. There was no artefactual quenching of the fluorescence by substances present in the Fenton-reaction mixture or in the artificial cerebrospinal fluid (aCSF) that might have influenced OH-TA quantification. A mathematical model based on kinetic considerations was developed. This explains the observed independence of the OH-TA concentration on the amount of TA2− present in aCSF as well as its dependence on TA2− concentration in potassium acetate buffer. A mechanism based on this model, involving complex formation between Fe(II), TA2− and H2O2, followed by an intra-molecular hydroxylation accompanied by an intra-molecular electron transfer was developed. The results are consistent with a reactive intermediate, which causes oxidative stress in vivo, not being a free hydroxyl radical, but a ferryl species or a “crypto” radical. The biological implications of these results are discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 103, Issue 1, January 2009, Pages 28–34
نویسندگان
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