کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1317897 976599 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction of arsenite with a zinc finger CCHC peptide: Evidence for formation of an As–Zn-peptide mixed complex
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Interaction of arsenite with a zinc finger CCHC peptide: Evidence for formation of an As–Zn-peptide mixed complex
چکیده انگلیسی

The interaction of arsenite with a Cys3His (CCHC) zinc finger model (34–51) HIV-1 nucleocapsid protein p7 (NCp7) peptide in the absence and presence of ZnII was studied using fluorescence spectroscopy, CD (circular dichroism) and ESI-MS (Electrospray Ionization Mass Spectrometry). We found that arsenic forms different complexes with the free peptide and the zinc finger peptide. In the former case the peptide conformation differed greatly from that of the zinc finger, whereas in the second case a mixed As–Zn-peptide complex was formed with partial preservation of zinc finger conformation. An apparent stability constant was estimated for the mixed As–Zn-peptide complex (K = 2083 M− 1 and 442 M− 1 at 25 °C and pHs 6 and 7, respectively). Our study also shows that the interaction of arsenic with the CCHC motif is facilitated by glutathione (GSH), through formation of a GS–As-peptide conjugate.

Fluorescence spectroscopy, circular dichroism and ESI-MS show the formation of As–ZF-peptides upon arsenite incubation with a Cys3His zinc finger.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 105, Issue 12, December 2011, Pages 1753–1758
نویسندگان
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