کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1317993 | 976625 | 2010 | 8 صفحه PDF | دانلود رایگان |
Iron(III) complexes [Fe(L)2]Cl (1–3), where L is monoanionic N-salicylidene-arginine (sal-argH for 1), hydroxynaphthylidene-arginine (nap-argH for 2) and N-salicylidene-lysine (sal-lysH for 3), were prepared and their DNA binding and photo-induced DNA cleavage activity studied. Complex 3 as its hexafluorophosphate salt [Fe(sal-lysH)2](PF6)·6H2O (3a) was structurally characterized by single crystal X-ray crystallography. The crystals belonged to the triclinic space group P-1. The complex has two tridentate ligands in FeN2O4 coordination geometry with two pendant cationic amine moieties. Complexes 1 and 2 with two pendant cationic guanidinium moieties are the structural models for the antitumor antibiotics netropsin. The complexes are stable and soluble in water. They showed quasi-reversible Fe(III)/Fe(II) redox couple near 0.6 V in H2O–0.1 M KCl. The high-spin 3d5-iron(III) complexes with μeff value of ∼5.9 μB displayed ligand-to-metal charge transfer electronic band near 500 nm in Tris–HCl buffer. The complexes show binding to Calf Thymus (CT) DNA. Complex 2 showed better binding propensity to the synthetic oligomer poly(dA)·poly(dT) than to CT-DNA or poly(dG)·poly(dC). All the complexes displayed chemical nuclease activity in the presence of 3-mercaptopropionic acid as a reducing agent and cleaved supercoiled pUC19 DNA to its nicked circular form. They exhibited photo-induced DNA cleavage activity in UV-A light and visible light via a mechanistic pathway that involves the formation of reactive hydroxyl radical species.
Iron(III) Schiff base complexes of arginine and lysine that are prepared and structurally characterized as netropsin mimics show AT-selective DNA binding and plasmid DNA cleavage activity in visible light.Figure optionsDownload as PowerPoint slide
Journal: Journal of Inorganic Biochemistry - Volume 104, Issue 4, April 2010, Pages 477–484