کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1318048 976641 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Iron-binding and anti-Fenton properties of baicalein and baicalin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Iron-binding and anti-Fenton properties of baicalein and baicalin
چکیده انگلیسی

Baicalein and baicalin, the major bioactive compounds found in the Chinese herb Scutellaria baicalensis, have been shown to be effective against cancer, bacterial infections and oxidative stress diseases. However, little is known about their mechanisms of action. To probe whether iron homeostasis modulation may play a role in their bioactivity, we have investigated their iron binding characteristics under physiologically relevant conditions. A 2:1 baicalein–ferrous complex was readily formed in 20 mM phosphate buffer, pH 7.2, with a binding constant ∼2–9 × 1011 M−2, whereas a 1:1 baicalein–ferric complex was formed, under the same conditions, with an apparent binding constant ∼1–3 × 106 M−1. Baicalein appears to bind the ferrous ion more strongly than ferrozine, a well known iron(II) chelator. Using 1 H NMR and Zn2+ and Ga3+ as probes, the iron-binding site on baicalein was elucidated to be at the O6/O7 oxygen atoms of the A-ring. No binding was observed for baicalin under the same NMR conditions. Furthermore, baicalein strongly inhibits the Fe-promoted Fenton chemistry via a combination of chelation and radical scavenging mechanism while baicalin can provide only partial protection against radical damage. These results indicate that baicalein is a strong iron chelator under physiological conditions and hence may play a vital role in modulating the body’s iron homeostasis. Modulation of metal homeostasis and the inhibition of Fenton chemistry may be one of the possible mechanisms for herbal medicine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 103, Issue 3, March 2009, Pages 326–332
نویسندگان
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