کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1321816 | 1499904 | 2013 | 6 صفحه PDF | دانلود رایگان |

Metallocarbonyl-labeled gold nanoparticles (displaying specific and intense absorption bands assigned to the stretching vibrations of the carbonyl ligands in IR spectroscopy) were derivatized with an anti-mouse IgG antibody to provide IR reporter immunoprobes that were characterized by several analytical techniques like ELISA, IR and transmission electron microscopy (TEM). These immunoprobes were able to detect a mouse monoclonal anti-staphylococcal enterotoxin A (SEA) antibody chemisorbed to a planar gold-coated glass sensor. The formation of the immune complex between metallocarbonyl–gold nanoparticle-antibody bioconjugate and anti-SEA antibody was assessed by mid-IR surface and atomic force microscopy (AFM) analysis.
Detection of mouse anti-staphylococcal enterotoxin A antibody chemisorbed onto a planar gold-coated glass sensor by mid-IR surface analysis using metallocarbonyl–gold-antibody bioconjugates.Figure optionsDownload as PowerPoint slideHighlights
► Metallocarbonyl-labeled AuNPs were derivatized with a goat anti-mouse IgG.
► Obtained bioconjugates were applied as IR reporter immunoprobes.
► Metallocarbonyl–gold-antibody immunoprobes were able to detect mouse antibody.
Journal: Journal of Organometallic Chemistry - Volume 734, 15 June 2013, Pages 32–37