کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1321816 1499904 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation of metallocarbonyl–gold-antibody bioconjugates for mid-IR optical immunosensing
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Preparation of metallocarbonyl–gold-antibody bioconjugates for mid-IR optical immunosensing
چکیده انگلیسی

Metallocarbonyl-labeled gold nanoparticles (displaying specific and intense absorption bands assigned to the stretching vibrations of the carbonyl ligands in IR spectroscopy) were derivatized with an anti-mouse IgG antibody to provide IR reporter immunoprobes that were characterized by several analytical techniques like ELISA, IR and transmission electron microscopy (TEM). These immunoprobes were able to detect a mouse monoclonal anti-staphylococcal enterotoxin A (SEA) antibody chemisorbed to a planar gold-coated glass sensor. The formation of the immune complex between metallocarbonyl–gold nanoparticle-antibody bioconjugate and anti-SEA antibody was assessed by mid-IR surface and atomic force microscopy (AFM) analysis.

Detection of mouse anti-staphylococcal enterotoxin A antibody chemisorbed onto a planar gold-coated glass sensor by mid-IR surface analysis using metallocarbonyl–gold-antibody bioconjugates.Figure optionsDownload as PowerPoint slideHighlights
► Metallocarbonyl-labeled AuNPs were derivatized with a goat anti-mouse IgG.
► Obtained bioconjugates were applied as IR reporter immunoprobes.
► Metallocarbonyl–gold-antibody immunoprobes were able to detect mouse antibody.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Organometallic Chemistry - Volume 734, 15 June 2013, Pages 32–37
نویسندگان
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