کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1322247 977189 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis, characterization and in vitro DNA binding studies of tin(IV) complexes of tert-butyl 1-(2-hydroxy-1-phenylethylamino)-3-methyl-1-oxobutan-2-yl carbamate
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Synthesis, characterization and in vitro DNA binding studies of tin(IV) complexes of tert-butyl 1-(2-hydroxy-1-phenylethylamino)-3-methyl-1-oxobutan-2-yl carbamate
چکیده انگلیسی

Tin(IV) complexes 1(a and b) and 2(a and b) of valine derived peptide derivatives were synthesized and characterized on the basis of elemental analysis, IR, 1H, 13C, 119Sn NMR, ESI-MS spectra and molar conductance measurements. The C–Sn–C angle was estimated from I3C and 1H NMR data 1J(119Sn, I3C) = 623 Hz; solution 2J(119Sn, 1H) = 93.04 Hz to be 149.9°. In vitro binding studies of complexes 1 and 2 under physiological conditions at room temperature with CT-DNA were carried out employing UV–visible, fluorescence, circular dichroism and viscometric studies. The binding affinity of the complexes was quantified by calculating the Kb values and it follows the order 2a > 1a > 2b > 1b. To further examine the specific mode of binding, the interaction of complexes 2(a and b) were carried out with 5′GMP and 5′TMP by using absorption and NMR (1H, 31P) spectroscopy. The supercoiled pBR322 plasmid DNA cleavage activity of the complexes was ascertained by gel electrophoresis assay. The complexes cleave supercoiled pBR322 plasmid DNA efficiently into its nicked form at micromolar concentrations.

Three dimensional structure of complex 1.Figure optionsDownload as PowerPoint slideHighlights
► Binding studies were carried out using various spectroscopic and biophysical methods.
► Results suggest a multifaceted mode of binding of the complexes with CT-DNA.
► Tethering of peptides to tin enhances the DNA cleavage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Organometallic Chemistry - Volume 696, Issue 22, 1 November 2011, Pages 3572–3579
نویسندگان
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