Antibacterial activities of ferrocenoyl- and cobaltocenium-peptide bioconjugates

The peptide and metallocene-peptide bioconjugates R-Arg-Arg-Phe-NH2, R-Phe-Arg-Phe-NH2 where R = H, Fe(Cp)(C5H4–CO), Co(Cp)(C5H4–CO)+ and R′-Gly-Trp-Arg-Arg-Phe-NH2, R′-Trp-Arg-Arg-Phe-NH2, where R′ = n-C5H11CO, Fe(Cp)(C5H4–CO), Co(Cp)(C5H4–CO)+, and Arg = l-arginine, Gly = l-glycine, Phe = l-phenylalanine, Trp = l-tryptophan were prepared by solid phase peptide synthesis (SPPS). The compounds were purified by RP-HPLC and characterized by ESI–MS and NMR spectroscopy. Antibacterial properties of the compounds were determined by minimum inhibitory concentration (MIC) tests against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.

Ferrocenoyl- and cobaltocenium-peptide bioconjugates of varing length (3–5 amino acids) were prepared by solid phase peptide synthesis and their antibacterial properties against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were determined by minimum inhibition concentration (MIC) tests.Figure optionsDownload as PowerPoint slide