کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
13472060 | 1846465 | 2020 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid and sensitive detection of diclazuril in chicken samples using a gold nanoparticle-based lateral-flow strip
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, we produced a sensitive and specific monoclonal antibody (mAb) against diclazuril based on a new hapten. The mAb, which belongs to the IgG2 subclass, had a 50%-inhibitory concentration of 0.36â¯ng/mL. A cross reactivity test revealed that the mAb had good specificity for diclazuril. A gold nanoparticle-based lateral-flow strip was assembled for the rapid screening of diclazuril in raw chicken breast and thigh samples. The strip assay had a visual limit of detection (LOD) of 2â¯Î¼g/kg and a cut-off value of 20â¯Î¼g/kg for diclazuril in chicken samples when evaluated with the naked eye. With the aid of a strip scan reader, the proposed assay obtained a quantitative measurement for diclazuril with an LOD of 1.08â¯Î¼g/kg. Therefore, this gold nanoparticle-based lateral-flow strip assay represents a potentially useful tool for on-site detection and rapid initial screening of diclazuril in chicken samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 312, 15 May 2020, 126116
Journal: Food Chemistry - Volume 312, 15 May 2020, 126116
نویسندگان
Zhongxing Wang, Xiaoling Wu, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu,