کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1355256 | 980833 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimized enzymatic preparation of 1-deoxy-d-xylulose 5-phosphate
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
The preparation of 1-deoxy-d-xylulose 5-phosphate, the key intermediate of MEP biosynthetic pathway for terpenoids by using recombinant 1-deoxy-d-xylulose 5-phosphate synthase of Rhodobacter capsulatus was optimized. The simple one-pot synthesis coupling with a newly established ion-exchange purification process affords the target compound with more than 80% yield and high purity (>95%). The procedure can also be employed to synthesize isotope labeled 1-deoxy-d-xylulose 5-phosphate by using isotope labeled starting materials.
1-deoxy-d-xylulose 5-phosphate was prepared in a one-pot method under optimized condition with more than 80% yield and high purity (>95%).Figure optionsDownload as PowerPoint slide
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic Chemistry - Volume 38, Issue 3, June 2010, Pages 120–123
Journal: Bioorganic Chemistry - Volume 38, Issue 3, June 2010, Pages 120–123
نویسندگان
Yue-Fei Zhou, Zhe Cui, Heng Li, Jie Tian, Wen-Yun Gao,