Diethanolamine Pd(II) complexes in bioorganic modeling as model systems of metallopeptidases and soybean lipoxygenase inhibitors

The reaction of PdCl2 with diethanolammonium chloride (DEAxHCl), in the molar ratio 1:2, affords the [HDEA]2[PdCl4] complex (1). The hydrolytic activity of the novel Pd(II) complex 1 was tested in reaction with N-acetylated L-histidylglycine dipeptide (AcHis-Gly). Complex 1, as well as earlier prepared trans-[PdCl2(DEA)2] complex (2), and DEA, as their precursor, were tested for their in vitro free radical scavenging activity. UV absorbance-based enzyme assays were done in order to evaluate their inhibitory activity of soybean lipoxygenase (LOX). Also, assays with superoxide anion radical were done. The scavenging activities of the complexes were measured and compared with those of their precursors and caffeic acid. Complex 2 exhibits the highest antioxidant activity and the highest inhibitory effect against the soybean LOX.

Complexes 1 and 2 were tested for their in vitro soybean lipoxygenase inhibitory and free radical scavenging activity. The hydrolytic reaction of the complex 1 with AcHis-Gly was investigated.Figure optionsDownload as PowerPoint slide