کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1356371 | 981112 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydrogen transfer pathways of the asymmetric reduction of α,β-unsaturated ketone mediated by baker's yeast
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The hydrogen transfer mechanism of cofactor reduction and recycling processes in the yeast reduction of α,β-unsaturated ketone was studied by using quantitative isotope tracing close to natural abundance measured by 2H NMR. In the reaction, the active cofactor is NADPH. The cofactor-transferred hydride attacks the β sp2 carbon of the enone carbonyl while water hydrogen is transferred to the α position. The reductant involved in the reaction depends on the quantity of yeast. When the amount of yeast is very large, the enzymes use preferentially certain unidentified substance stored in the yeast cells rather than the added glucose as electron donor. In this case, the hydrogen transferred by the cofactor is mainly of water origin. When the yeast amount is low, the added glucose is more efficiently used by the enzymes as electron donor and its hydrogen atoms bound to C-1 and C-3 are delivered to the substrate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic Chemistry - Volume 34, Issue 3, June 2006, Pages 158-166
Journal: Bioorganic Chemistry - Volume 34, Issue 3, June 2006, Pages 158-166
نویسندگان
Yuan Chu, Ben Li Zhang, Virginie Silvestre, Jin Pei Cheng,