کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1358336 981336 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Probing the stabilizing effects of modified nucleotides in the bacterial decoding region of 16S ribosomal RNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Probing the stabilizing effects of modified nucleotides in the bacterial decoding region of 16S ribosomal RNA
چکیده انگلیسی

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The bacterial decoding region of 16S ribosomal RNA has multiple modified nucleotides. In order to study the role of N4,2′-O-dimethylcytidine (m4Cm), the corresponding phosphoramidite was synthesized utilizing 5′-silyl-2′-ACE chemistry. Using solid-phase synthesis, m4Cm, 5-methylcytidine (m5C), 3-methyluridine (m3U), and 2′-O-methylcytidine (Cm) were site-specifically incorporated into small RNAs representing the decoding regions of different bacterial species. Biophysical studies were then used to provide insight into the stabilizing roles of the modified nucleotides. These studies reveal that methylation of cytidine and uridine has different effects. The same modifications at different positions or sequence contexts within similar RNA constructs also have contrasting roles, such as stabilizing or destabilizing the RNA helix.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 10, 15 May 2013, Pages 2720–2726
نویسندگان
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