کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1358589 | 981350 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells.
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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 20, Issue 13, 1 July 2012, Pages 3985–3990
Journal: Bioorganic & Medicinal Chemistry - Volume 20, Issue 13, 1 July 2012, Pages 3985–3990
نویسندگان
Yuka Miyano, Senko Tsukuda, Ippei Sakimoto, Ryo Takeuchi, Satomi Shimura, Noriyuki Takahashi, Tomoe Kusayanagi, Yoichi Takakusagi, Mami Okado, Yuki Matsumoto, Kaori Takakusagi, Toshifumi Takeuchi, Shinji Kamisuki, Atsuo Nakazaki, Keisuke Ohta,