کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1358934 | 981373 | 2013 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Oligonucleotide probes containing pyrimidine analogs reveal diminished hydrogen bonding capacity of the DNA adduct O6-methyl-G in DNA duplexes Oligonucleotide probes containing pyrimidine analogs reveal diminished hydrogen bonding capacity of the DNA adduct O6-methyl-G in DNA duplexes](/preview/png/1358934.png)
Oligonucleotide hybridization probes containing nucleoside analogs offer a potential strategy for binding specific DNA sequences that bear pro-mutagenic O6-G alkylation adducts. To optimize O6-Me-G-targeting probes, an understanding of how base pairs with O6-Me-G are stabilized is needed. In this study, we compared the ability of O6-Me-G and G to hydrogen bond with three pyrimidine-like nucleobases (Z, 4-thio-U, and 3-deaza-C) bearing varied hydrogen bond donor and acceptor groups. We found that duplexes containing the pyrimidine analog nucleoside:G pairs were more thermodynamically stable than those containing pyrimidine analog nucleoside:O6-alkyl-G pairs. Thus, hydrogen bonding alone was not sufficient to impart selectivity to probes that target O6-G alkylation adducts in DNA.
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Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 20, 15 October 2013, Pages 6212–6216