کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1359315 981399 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A concise preparation of the fluorescent amino acid l-(7-hydroxycoumarin-4-yl) ethylglycine and extension of its utility in solid phase peptide synthesis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
A concise preparation of the fluorescent amino acid l-(7-hydroxycoumarin-4-yl) ethylglycine and extension of its utility in solid phase peptide synthesis
چکیده انگلیسی

Incorporation of the unnatural amino acid l-(7-hydroxycoumarin-4-yl)ethylglycine (7-HC) is a powerful and reliable approach for the preparation of fluorescently labeled proteins. The growing popularity of this valuable amino acid prompted us to pursue an improved protocol for its synthetic preparation. The optimized procedure here described provides ready access to multi-gram quantities of 7-HC. Also reported is an extension of the utility of 7-HC in the generation of a protected building block suitable for use in solid phase peptide synthesis. The building block was successfully incorporated at various positions in a series of model peptides, including analogues of the cell penetrating HIV-Tat peptide, further illustrating the utility of this unique amino acid.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 21, Issue 2, 15 January 2013, Pages 553–559
نویسندگان
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