کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1359808 981415 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel fluorescent ceramide derivatives for probing ceramidase substrate specificity
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Novel fluorescent ceramide derivatives for probing ceramidase substrate specificity
چکیده انگلیسی

Ceramidases are key regulators of cell fate. The biochemistry of different ceramidases and of their substrate ceramide appears to be complex, mainly due to specific biophysical characteristics at the water-membrane interface. In the present study, we describe the design and synthesis of a set of fluorescently labeled ceramides as substrates for acid and neutral ceramidases. For the first time we have replaced the commonly used polar NBD-dye with the lipophilic Nile Red (NR) dye. Analysis of kinetic data reveal that although both the dyes do not have any noticeable preference for the substitution at acyl or sphingosine (Sph) part in ceramide towards hydrolysis by acid ceramidase, the ceramides with acyl-substituted NBD and Sph-substituted NR dyes have been found to be a better substrate for neutral ceramidase.

In this study we have synthesized novel Nile Red (NR)-labeled ceramides as substrates of acid and neutral ceramidases. Structure–activity-relationship and kinetic studies with NR- and commercially available NBD-ceramides provide insights into their substrate specificities toward recombinant acid and neutral ceramidases.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 20, Issue 20, 15 October 2012, Pages 6154–6161
نویسندگان
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