کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1360003 981422 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis and hybridization of 2′-O-methyl-RNAs incorporating 2′-O-carbamoyluridine and unique participation of the carbamoyl group in U–G base pair
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Synthesis and hybridization of 2′-O-methyl-RNAs incorporating 2′-O-carbamoyluridine and unique participation of the carbamoyl group in U–G base pair
چکیده انگلیسی

2′-O-Carbamoyluridine (Ucm) was synthesized and incorporated into DNAs and 2′-O-Me-RNAs. The oligonucleotides incorporating Ucm formed less stable duplexes with their complementary and Ucm–U, Ucm–C single-base mismatched DNAs and RNAs in comparison with those without the carbamoyl group. On the contrary, the Tm analyses revealed that the duplexes with a mismatched Ucm–G base pair showed almost the same thermostability as the corresponding unmodified duplexes. Molecular dynamics (MD) simulations of the Ucm-modified 2′-O-Me-RNA/RNA duplexes with Ucm–G mismatched base pair suggested that the carbamoyl group could participate in the Ucm–G base pair by an additional intermolecular hydrogen bond between the carbamoyl oxygen and the H2 of the guanine base.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 17, Issue 20, 15 October 2009, Pages 7275–7280
نویسندگان
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