Engineered production of iso-migrastatin in heterologous Streptomyces hosts

Glutarimide-containing polyketides such as migrastatin (MGS) are well known for their ability to inhibit tumor cell migration. We have previously shown that MGS is derived from iso-migrastatin (iso-MGS) via a H2O-mediated ring-expansion rearrangement. A bacterial artificial chromosome (BAC) library of Streptomyces platensis NRRL18993, an iso-MGS producer, was constructed. From this library, pBS11001, a BAC clone harboring the intact iso-MGS biosynthetic gene cluster, was identified. Mobilization of pBS11001 into five heterologous Streptomyces hosts afforded recombinant strains, SB11001, SB11002, SB11003, SB11004, and SB11005, respectively. Under a standard set of media and fermentation conditions, the recombinant strains all produced the same profile of iso-MGS as that of S. platensis NRRL18993. These findings highlight the strength and flexibility of the BAC-based technology for natural product production and engineering in heterologous Streptomyces model hosts.

Production of iso-migrastatin in recombinant strains SB11001, SB11002, SB11003, SB11004, and SB11005 has been accomplished by BAC-mediated expression of the iso-migrastatin biosynthetic gene cluster from Streptomyces platensis NRRL18993 in five heterologous Streptomyces hosts.Figure optionsDownload as PowerPoint slide