کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1360294 981432 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Efficient solid-phase synthesis of oligodeoxynucleotides having a 5′-terminal 2,2,7-trimethylguanosine pyrophosphate linkage
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Efficient solid-phase synthesis of oligodeoxynucleotides having a 5′-terminal 2,2,7-trimethylguanosine pyrophosphate linkage
چکیده انگلیسی

A 5′-terminal 2,2,7-trimethylguanosine (TMG) cap structure plays an important role in transport of TMG-capped snRNAs from the cytoplasm to the nucleus in living cells. This property can be expected to be used for carrying artificial nucleic acids into the nucleus in gene therapy. In this paper, a new TMG-capping reagent 1 was developed and used for the synthesis of oligodeoxynucleotides having a 5′-terminal TMG-capped pyrophosphate linkage (m32,2,7G5′pp-DNA) lacking one phosphate group using a silyl-type linker on polymer supports.

A new TMG-capping reagent 1 was developed and used for the synthesis of oligodeoxynucleotides having a 5′-terminal TMG-capped pyrophosphate linkage (m32,2,7G5′pp-DNA) lacking one phosphate group by using a silyl-type linker on polymer supports.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 17, Issue 13, 1 July 2009, Pages 4819–4824
نویسندگان
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