کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1360531 981438 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hybridization-dependent fluorescence of oligodeoxynucleotides incorporating new pyrene-modified adenosine residues
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Hybridization-dependent fluorescence of oligodeoxynucleotides incorporating new pyrene-modified adenosine residues
چکیده انگلیسی

We report the synthesis and properties of oligonucleotides incorporating N6-[N-(pyren-1-ylmethyl)carbamoyl]-deoxyadenosine (dApymcm). We designed the ODN which incorporated two consecutive dApymcm residues. It was revealed that on hybridization with the target DNA and RNA oligomers, the fluorescence spectra of ODNs having two consecutive dApymcm molecules near the 5′-terminal position can change from the pyrene monomer emission to the excimer, depending on the chain length of the target DNA and RNA. These results indicated that dApymcm-modified ODNs can be used as interesting hybridization sensors that are sensitive to the size of the target strand.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 16, Issue 17, 1 September 2008, Pages 8287–8293
نویسندگان
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