کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1360723 981446 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study of the base discrimination ability of DNA and 2′-O-methylated RNA oligomers containing 2-thiouracil bases towards complementary RNA or DNA strands and their application to single base mismatch detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Study of the base discrimination ability of DNA and 2′-O-methylated RNA oligomers containing 2-thiouracil bases towards complementary RNA or DNA strands and their application to single base mismatch detection
چکیده انگلیسی

Precise detection of target DNA and RNA sequences using chemically modified oligonucleotides is of crucial importance in gene analysis and gene silence. The hybridisation and base discrimination abilities of oligonucleotides containing 2′-O-methyl-2-thiouridine (s2Um) in homo- and hetero-duplexes composed of DNA and RNA strands have been studied in detail. When s2Um was incorporated into RNA or DNA strands, the hybridisation and base discrimination abilities of the modified RNA or DNA oligomers towards the complementary RNA strands were superior to those of the corresponding unmodified oligomers. On the other hand, their base discrimination abilities towards complementary DNA strands were almost the same as those of the unmodified ones. The base discrimination abilities of 2-thiouracil base-containing oligonucleotide probes on slide glass plates were also studied. These modified probes exhibited efficient detection of mismatched base pairing.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 16, Issue 11, 1 June 2008, Pages 6034–6041
نویسندگان
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