کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1360822 981448 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Anionic polymer, poly(methyl vinyl ether–maleic anhydride)-coated beads-based capture of human influenza A and B virus
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Anionic polymer, poly(methyl vinyl ether–maleic anhydride)-coated beads-based capture of human influenza A and B virus
چکیده انگلیسی

An anionic magnetic beads-based method was developed for the capture of human influenza A and B viruses from nasal aspirates, allantoic fluid and culture medium. A polymer, poly(methyl vinyl ether–maleic anhydride) [poly(MVE-MA)], was used to endow magnetic beads with a negative charge and bioadhesive properties. After incubation with samples containing human influenza virus, the beads were separated from supernatants by applying a magnetic field. The absorption of the virus by the beads was confirmed by hemagglutinin assay, immunochromatography, Western blotting, egg infection, and cell infection. Successful capture was proved using 5 H1N1 influenza A viruses, 10 H3N2 influenza A viruses, and 6 influenza B viruses. Furthermore, the infectivity in chicken embryonated eggs and Madin–Darby canine kidney (MDCK) cells of the captured human influenza virus was similar to that of the total viral quantity of starting materials. Therefore, this method of capture using magnetic beads coated with poly(MVE-MA) can be broadly used for the recovery of infectious human influenza viruses.

Poly(methyl vinyl ether–maleic anhydride) [poly(MVE-MA)] is an anionic and bioadhesive polymer. Poly(MVE-MA) in water forms highly polar polymeric free acids, which are highly negatively charged. Coating magnetic beads with poly(MVE-MA) facilitated the capture and concentration of human influenza viruses.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 17, Issue 2, 15 January 2009, Pages 752–757
نویسندگان
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