کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1361231 981459 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of chemical, radiochemical and optical properties of a dual-labeled MMP-9 targeting peptide
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Characterization of chemical, radiochemical and optical properties of a dual-labeled MMP-9 targeting peptide
چکیده انگلیسی

Optical imaging possesses similar sensitivity to nuclear imaging and has led to the emergence of multimodal approaches with dual-labeled nuclear/near-infrared (NIR) agents. The growing impact of 68Ga (t1/2 = 68 min) labeled peptides on preclinical and clinical research offers a promising opportunity to merge the high spatial resolution of NIR imaging with the clinically-accepted positron emission tomography (PET). Previously, dual-labeled agents have been prepared with longer-lived radiometals and showed no detrimental effects on optical properties as a result of radiolabeling. In this study, we selected a peptide (M2) that targets MMP-2/9 and is dual-labeled with IRDye 800CW and 68Ga. Since 68Ga chelation typically requires low pH (3.5–4) and elevated heating temperatures (95 °C), we sought to evaluate the impact of 68Ga labeling on the optical properties of M2. An efficient method for preparation of 68Ga-M2 was developed and reaction conditions were optimized. Stability studies in PBS, DTPA, and serum were performed and high levels of intact agent were evident under each condition. The addition of multiple reporters to a targeting agent adds further complexity to the characterization and validation and thus requires not only testing to ensure the agent is stable chemically and radiochemically, but also optically. Therefore, fluorescence properties were evaluated using a spectrofluorometer as well as by fluorescence detection via HPLC. It was determined that 68Ga-labeling conditions did not impair the fluorescent properties of the agent. The agent was then used for in vivo imaging in a mouse model of heterotopic ossification (HO) with activated MMP-9 expression as an early biomarker which precedes mineralization. Although 68Ga-complexation greatly reduced binding affinity of the peptide and negated tracer uptake on PET, NIR imaging showed consistent fluorescent signal that correlated to MMP-9 expression. This attests to the feasibility of using 68Ga/NIR for dual-labeling of other peptides or small molecules for multimodality molecular imaging.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 12, 15 June 2011, Pages 3769–3776
نویسندگان
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