کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1361774 | 981471 | 2008 | 6 صفحه PDF | دانلود رایگان |

A new bis-pyrene-labeled oligonucleotide probe (BP-probe) has been designed for the detection of a single base mismatch in single strand (ss) DNA as a target. The sequence of BP-probe was chosen to form stem-loop structure similar to a molecular beacon (MB-probe), yielding bis-pyrene-labeled molecular beacon (BP–MB-probe). Partially double stranded (ds) BP–MB-probes were prepared by complexation with oligonucleotides whose sequences are complementary to the loop segment but not to the stem and exchangeable with the target DNA. The partially ds BP–MB-probes were shown to exhibit monomer fluorescence as major fluorescence, while the ss BP–MB-probe in the stem-loop form displays strong excimer fluorescence. The strand exchange reactions between partially ds BP–MB-probe and target ss DNA in the presence of cationic comb-type copolymer as a catalyst were monitored by the excimer fluorescence changes. The existence of a mismatched base can be determined by the slower PASE rates compared with fully matched DNA.
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Journal: Bioorganic & Medicinal Chemistry - Volume 16, Issue 1, 1 January 2008, Pages 78–83