کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1362274 981482 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biosynthesis of acaterin: Mechanism of the reaction catalyzed by dehydroacaterin reductase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Biosynthesis of acaterin: Mechanism of the reaction catalyzed by dehydroacaterin reductase
چکیده انگلیسی

Dehydroacaterin reductase is an enzyme which catalyzes the final step of acaterin biosynthesis, that is, the reduction of the C-4/C-5 double bond of dehydroacaterin. The mechanism of the reduction was investigated with a cell-free preparation obtained from the acaterin-producing microorganism, Pseudomonas sp. A 92. Incubation of dehydroacaterin in the presence of [4,4-2H2]NADPH or D2O followed by 2H NMR analysis of the resulting acaterin revealed that the deuterium atom from NADPH was incorporated into the C-5 position of acaterin, while the deuterium atom from D2O was introduced into the C-4 position. It was further demonstrated that the pro-R hydrogen at C-4 of NADPH was stereospecifically utilized in this reduction.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 14, Issue 18, 15 September 2006, Pages 6404–6408
نویسندگان
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