A facile and effective synthesis of dinucleotide 5′-triphosphates

We report on the successful synthetic procedure for the conversion of 5′-monophosphorylated 2′-deoxydinucleotides into their 5′-triphosphate derivatives in satisfactory to excellent yields. The activation of the terminal phosphate group was achieved under the Mukaiyama conditions in the presence of a nucleophilic catalyst. The reaction conditions (solvent, counter ions, activation time and reagent excess) were optimized for all dinucleotides.

Successful synthetic procedure for the conversion of 5′-monophosphorylated 2′-deoxydinucleotides into their 5′-triphosphate derivatives in satisfactory to excellent yields.Figure optionsDownload as PowerPoint slide