کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1363083 981502 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Binding of lectins to DNA micro-assemblies: Modification of nucleo-cages with lactose-conjugated psoralen
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Binding of lectins to DNA micro-assemblies: Modification of nucleo-cages with lactose-conjugated psoralen
چکیده انگلیسی

Spherical DNA micro-assemblies appended with lactose units (Lactose-nucleo-cages, Lac-NC) are newly developed. DNA spherical assemblies self-assembled from suitably designed three oligodeoxyribonucleotides (ODNs) 1–3 were cross-linked by lactose-conjugated psoralen derivative 4. Confocal laser scanning fluorescence microscopy (CLSM) observation of Lac-NC shows that rhodamine labeled peanut lectin (Rho-PNA: a galactose-specific lectin) binds to lactose-modified nucleo-cages with higher affinity compared to that of unmodified nucleo-cages. Binding isotherm experiments indicated that the apparent affinity constant of Rho-PNA to Lac-NC was in the order of 105 M−1.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 15, Issue 12, 15 June 2007, Pages 4311–4317
نویسندگان
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