Chemically modified oligonucleotides with efficient RNase H response

Schematic model of RNase H enzyme (in green) with its cleaving site in red. The horizontal arrow indicates direction of movement of the enzyme and the star indicates P32 labelling of the RNA to allow visualization of RNA fragments by gel electrophoresis. The study shows that it is possible to introduce modified nucleotides in an antisense oligonucleotide across RNA cleavage sites, which maintain or even improve RNA cleavage by RNaseH.