Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology

The synthesis and initial evaluation of novel chromogenic substrates with potential in the detection and differentiation of cultured bacterial colonies are described. The substrates were readily hydrolysed by specific aminopeptidase activity to release the chromogen, 9-(4′-aminophenyl)-10-methylacridinium salt, which provided a clear visual indication of the presence of the corresponding bacteria.

The synthesis and evaluation of novel chromogenic peptidase substrates 4a–g with good species specificity are described.Figure optionsDownload as PowerPoint slide