کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1366567 981595 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aptazyme-based riboswitches as label-free and detector-free sensors for cofactors
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Aptazyme-based riboswitches as label-free and detector-free sensors for cofactors
چکیده انگلیسی

We constructed a label-free and detector-free aptazyme-based riboswitch sensor for detecting the cofactor of the aptazyme. This riboswitch, which usually suppresses the gene expression with its anti-RBS sequence bound to the RBS of its own mRNA (OFF), activates the translation only when a cofactor is added to release the anti-RBS sequence from itself as a result of cofactor-induced self-cleavage by the aptazyme (ON). The rationally optimized one with β-galactosidase as a reporter gene enabled us to detect the cofactor of the aptazyme visibly with high ON/OFF efficiency.

We constructed a label-free and detector-free aptazyme-based riboswitch sensor for detecting the cofactor of the aptazyme.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 17, Issue 11, 1 June 2007, Pages 3156–3160
نویسندگان
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