The design and synthesis of YC-1 analogues as probes for soluble guanylate cyclase

Soluble guanylate cyclase (sGC) is highly activated in the presence of both YC-1 (1-benzyl-3-(5′-hydroxymethyl-2′-furyl)-indazole) and CO. In this report, the design, synthesis, and activity (i.e., sGC activation) of photolabile analogues of 3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1) are presented. Initial results with 6-azido-3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole led to the synthesis of a tritium-labeled analogue. When photoactivated, this analogue labeled the α-subunit of sGC.

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