Synthesis of 13C7-labeled iodoacetanilide and application to quantitative analysis of peptides and a protein by isotope differential mass spectrometry

13C7-Labeled iodoacetanilide has been synthesized for specific labeling of sulfhydryl groups of cysteine residues and has been successfully applied to quantitative analysis of peptides and a commercial protein in combination with 13C-unlabeled iodoacetanilide and a MALDI mass spectrometer. Subsequent tandem mass spectrum analysis revealed that 13C7-labeled iodoacetanilide remained intact during the collision-induced dissociation (CID) conditions.

13C7-Labeled and unlabeled iodoacetanilide that covalently react with cysteine residues have been synthesized and applied to quantitative analysis of peptides and a protein.Figure optionsDownload as PowerPoint slide