Carrier PNA for shRNA delivery into cells

A peptide nucleic acid (PNA)-cell-penetrating peptide (CPP) conjugate (carrier PNA) was used as ‘bridge-builder’ to connect a CPP with an shRNA. The carrier PNA successfully formed a hybrid with an shRNA bearing complementary dangling bases and the shRNA was introduced into cells by the carrier PNA, and RNAi was induced by the shRNA.

A peptide nucleic acid conjugated to a cell-penetrating peptide (carrier PNA) sequence-specifically binds to an shRNA bearing complementary dangling bases, and the carrier PNA delivers the shRNA effectively into cells.Figure optionsDownload as PowerPoint slide