کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1372788 981881 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Substituent effect on the preferred DNA binding mode and affinity of a homologous series of naphthalene diimides
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Substituent effect on the preferred DNA binding mode and affinity of a homologous series of naphthalene diimides
چکیده انگلیسی

A combination of isothermal titration calorimetry (ITC), topoisomerase I DNA unwinding assays, and ethidium bromide displacement studies were employed to investigate the binding of a homologous series of naphthalene diimides (NDI) to DNA. Our results suggest that the nature of the substituent plays a significant role in both the preferred binding mode and relative binding affinity of the compounds of this study. Only intercalative-type binding (K = 15 ± 3 × 106 M−1) was observed for the NDI with the smallest substituent (trimethyl-ethylamino), while larger members of the series (diethylmethyl-, dipropylmethyl- and dibutylmethyl-ethylamino substituents) adopted an additional binding mode of higher affinity (K1 = 31 − 78 × 106 M−1).

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 21, Issue 14, 15 July 2011, Pages 4288–4291
نویسندگان
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