کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1372826 | 981882 | 2008 | 5 صفحه PDF | دانلود رایگان |
The synthesis and biological evaluation of a new cap analog, which is modified at the C2′ and C3′ positions of N7-methylguanosine is reported. The new cap analog, P1-2′,3′-isopropylidene, 7-methylguanosine-5′P3-guanosine-5′triphosphate was assayed with respect to its effects on efficiency of incorporation into RNAs during in vitro transcription, and intracellular stability and translational activity of its 5′-capped mRNAs, upon transfection into HeLa cells. The intracellular stability of 5′-capped and uncapped full length test mRNAs was measured by using a real-time RT-PCR assay. The RNA with the 5′-modified cap was found to be ∼1.7 times more stable than the RNA with the 5′-standard cap and ∼2.5 times more stable than the uncapped control RNA. The translational efficiency was monitored by measuring the luciferase activity of a variety of in vitro synthesized and capped RNAs coding for a luciferase fusion protein after transfection into HeLa cells. The RNA capped with the 2′,3′-isopropylidene substituted analog, (m7,2′,3′-isopropylideneG[5′]ppp[5′]G), was translated the most efficiently, with ∼ 2.9-fold more activity than the standard cap (m7G[5′]ppp[5′]G). The observed increase in the level of protein synthesis is likely resulted as a consequence of exclusively forward capped transcripts and increased cellular stability of the 5′-modified capped mRNA (Poly A).
The synthesis and biological evaluation of C2′ and C3′ disubstituted at m7Guo cap analog, that is, m7,2′,3′-isopropylideneG[5′]ppp[5′]G is reported.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 17, 1 September 2008, Pages 4828–4832