Keywords: T7 RNA پلیمراز; African horse sickness virus; Orbivirus; Reverse genetics; dsRNA; Reoviridae; Virus rescue; T7 RNA polymerase; Reassortment;
مقالات ISI T7 RNA پلیمراز (ترجمه نشده)
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Expression of T7-based constructs in tobacco cells
Keywords: T7 RNA پلیمراز; T7 promoter; T7 RNA polymerase; Recombinant protein expression; Plant;
Construction of synthetic T7 RNA polymerase expression systems
Keywords: T7 RNA پلیمراز; T7 RNA polymerase; Synthetic circuits; Feedback systems; Gene expression;
An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening
Keywords: T7 RNA پلیمراز; Ebola virus; Filoviruses; Minigenome system; RNA polymerase II; T7 RNA polymerase; Antiviral drug screening;
Efficient genetic approaches for improvement of plasmid based expression of recombinant protein in Escherichia coli: A review
Keywords: T7 RNA پلیمراز; E. coli; Escherichia coli; DsbC; disulphide bond C; ORI; origin of replication; SUMO; Small Ubiquitin-like modifier; PSK; post segregational killing; GST; glutathione S-transferase; ORT; operator-repressor titration; NusA; N-utilization substance A; T7 RN
Mechanism of pyrophosphate ion release in T7 RNA polymerase revealed by free energy simulations
Keywords: T7 RNA پلیمراز; Molecular dynamics simulation; T7 RNA polymerase; PPi release; Potential of mean force;
Applications of PLOR in labeling large RNAs at specific sites
Keywords: T7 RNA پلیمراز; NMR; nuclear magnetic resonance; PLOR; position-selective labeling of RNA; T7 RNAP; T7 RNA polymerase; nt; nucleotides; smFRET; single molecule Förster resonance-energy transfer; SPE; solid-phase extraction; PCR; polymerase chain reaction; NOESY; nuclear
Development of a highly specific and sensitive cadmium and lead microbial biosensor using synthetic CadC-T7 genetic circuitry
Keywords: T7 RNA پلیمراز; CadC; Heavy metal ions; Microbial biosensor; Microfluidic device; T7 RNA polymerase;
CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production
Keywords: T7 RNA پلیمراز; CRISPR; Clustered regularly interspaced short palindromic repeats; CRISPRi; Clustered regularly interspaced short palindromic repeats interference; MVA; mevalonate; sgRNA; single guide RNA; dCas9; inactive Cas9; IPP; isopentenyl diphosphate; DMAPP; dimeth
Establishment of an entirely plasmid-based reverse genetics system for Bluetongue virus
Keywords: T7 RNA پلیمراز; Bluetongue virus; Orbivirus; DsRNA; Reverse genetics; Reverse genetics plasmid; T7 RNA polymerase; Genome modification; Reassortment;
Construction of a host-independent T7 expression system with small RNA regulation
Keywords: T7 RNA پلیمراز; T7 RNA Polymerase; T7 Promoter; Antisense RNA; Host-independent expression system
A plasmid-based reverse genetics system for mammalian orthoreoviruses driven by a plasmid-encoded T7 RNA polymerase
Keywords: T7 RNA پلیمراز; Reovirus; Reverse genetics; T7 RNA polymerase; Eukaryotic expression plasmid;
Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin
Keywords: T7 RNA پلیمراز; 2,3-Butanediol; Acetoin; xylF; T7 RNA polymerase; Butanediol dehydrogenase; Diacetyl acetoin reductase;
Bacteriophage T7 RNA polymerase-based expression in Pichia pastoris
Keywords: T7 RNA پلیمراز; Recombinant protein production; Pichia pastoris; T7 RNA polymerase; Internal ribosomal entry site; Pichia pastoris autonomous replicating sequence;
Evolution of an inhibitory RNA aptamer against T7 RNA polymerase
Keywords: T7 RNA پلیمراز; RNA aptamer; T7 RNA polymerase; SELEXRNAP, RNA polymerase; SELEX, systematic evolution of ligands by exponential enrichment
High-throughput screening method for promoter activity using bead display and a ligase ribozyme
Keywords: T7 RNA پلیمراز; In vitro promoter screening; Emulsion polymerase chain reaction; Fluorescence activated cell sorting (FACS); Ribozyme; T7 RNA polymerase
Reverse genetics for mammalian reovirus
Keywords: T7 RNA پلیمراز; Reovirus; Reverse genetics; dsRNA; T7 RNA polymerase; Reassortment
Optimized method for the synthesis and purification of adenosine – Folic acid conjugates for use as transcription initiators in the preparation of modified RNA
Keywords: T7 RNA پلیمراز; Folic acid; Adenosine monophosphate (AMP); Affi-gel boronate; In vitro transcription; T7 RNA polymerase; RNA labeling
Evaluation of small ligand–protein interactions by using T7 RNA polymerase with DNA-modified ligand
Keywords: T7 RNA پلیمراز; Ligand–protein interaction; T7 RNA polymerase; DNA tag; Transcription
An improved reverse genetics system for mammalian orthoreoviruses
Keywords: T7 RNA پلیمراز; Reverse genetics; Reovirus; dsRNA; T7 RNA polymerase; Reassortment
A novel T7 RNA polymerase dependent expression system for high-level protein production in the phototrophic bacterium Rhodobacter capsulatus
Keywords: T7 RNA پلیمراز; Rhodobacter; Heterologous expression; T7 RNA polymerase; Expression system; Photosynthetic bacteria; Broad-host-range plasmid; High-level expression; Protein production
Development of a hamster kidney cell line expressing stably T7 RNA polymerase using retroviral gene transfer technology for efficient rescue of infectious foot-and-mouth disease virus
Keywords: T7 RNA پلیمراز; Foot-and-mouth disease virus; Reverse genetics; Stable BHK-21 cell line; T7 RNA polymerase
Transient inhibition of foot-and-mouth disease virus replication by siRNAs silencing VP1 protein coding region
Keywords: T7 RNA پلیمراز; Foot-and-mouth disease virus; Small interfering RNA; T7 RNA polymerase; Flow cytometry; Real-time quantitative PCR
Synthesis and application of a new 2′,3′-isopropylidene guanosine substituted cap analog
Keywords: T7 RNA پلیمراز; Cap analog; T7 RNA polymerase; Capping efficiency; In vitro transcription; mRNA stability; Translation efficiency; Luciferase activity; HeLa cells
Non-invasive, ultra-sensitive, high-throughput assays to quantify rare biomarkers in the blood
Keywords: T7 RNA پلیمراز; T7 RNA polymerase; RiboGreen; ELISA; IDAT; FACTT; Biomarker; Blood test; High-throughput; HER2; Prion
Facilitated in vivo synthesis of ribonucleic acid and protein via T7 RNA polymerase
Keywords: T7 RNA پلیمراز; T7 RNA polymerase; Stable clone; In vivo synthesis; siRNA; Ribozyme; Protein production
Imaging and analysis of transcription on large, surface-mounted single template DNA molecules
Keywords: T7 RNA پلیمراز; Promoter detection; Functionalized surfaces; Fluorescence microscopy; T7 RNA polymerase; AFM;
Transcription of DNA containing the 5-guanidino-4-nitroimidazole lesion by human RNA polymerase II and bacteriophage T7 RNA polymerase
Keywords: T7 RNA پلیمراز; TCR; transcription-coupled repair; hRNAPII; human RNA polymerase II; T7RNAP; T7 RNA polymerase; NI; 5-guanidino-4-nitroimidazole; ONOOâ; peroxynitrite; NO; nitric oxide; O2â; superoxide anion; QM; quantum mechanics; MD; molecular dynamics; DNAP; DNA p
One step engineering of T7-expression strains for protein production: Increasing the host-range of the T7-expression system
Keywords: T7 RNA پلیمراز; T7 expression; Protein expression; T7 RNA polymerase; T7 lysozyme
Functional Architecture of T7 RNA Polymerase Transcription Complexes
Keywords: T7 RNA پلیمراز; RNAP, RNA polymerase; T-strand, template strand; NT-strand, non-template strand; IC, initiation complex; EC, elongation complex; Fe-BABE, iron (S)-1-(p-bromoacetamidobenzyl)ethylenediamine-tetraacetate; WT, wild-typeRNA polymerase; T7 RNA polymerase; DNA
Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers
Keywords: T7 RNA پلیمراز; APRT; adenine phosphoribosyltransferase; BLE; gene encoding for phleomycin binding protein; CAM A; calmodulin A gene; CAM IR; calmodulin intergenic region; EGFP; gene encoding for enhanced green fluorescent protein; HYG; hygromycin resistance gene; IR; in
Transchip: Single-molecule detection of transcriptional elongation complexes
Keywords: T7 RNA پلیمراز; Single-molecule; Transcription; Elongation complex; T7 RNA polymerase; Promoter mapping; Automated fluorescence microscopy
Inhibition of hepatitis C virus RNA replication by short hairpin RNA synthesized by T7 RNA polymerase in hepatitis C virus subgenomic replicons
Keywords: T7 RNA پلیمراز; Hepatitis C virus; RNAi; Synthesized shRNA; T7 RNA polymerase; IFN; PKR; 2′-5′OAS; Hepatitis C virus subgenomic replicons
Coupled cytoplasmic transcription-and-translation—a method of choice for heterologous gene expression in Xenopus oocytes
Keywords: T7 RNA پلیمراز; Expression technique; Xenopus oocytes; T7 RNA polymerase; Luciferase
Mapping the Conformation of the Nucleic Acid Framework of the T7 RNA Polymerase Elongation Complex in Solution Using Low-energy CD and Fluorescence Spectroscopy
Keywords: T7 RNA پلیمراز; transcription; 2-aminopurine; T7 RNA polymerase; circular dichroism; fluorescenceRNAP, RNA polymerase; 2-AP, 2-aminopurine
Translocation by T7 RNA Polymerase: A Sensitively Poised Brownian Ratchet
Keywords: T7 RNA پلیمراز; T7 RNA polymerase; translocation; transcription elongation; Brownian ratchet; transcription dynamicsRNAP, RNA polymerase; T-strand, template strand; NT-strand, non-template strand; IC, initiation complex; EC, elongation complex; Fe-BABE, iron (S)-1-(p-bro
Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects
Keywords: T7 RNA پلیمراز; Bloodstream-form; Functional genomics; GFP; Leaky; Position effect; RNAi; Transfection; T7 RNA polymerase;
Human protein tau represses DNA replication in vitro
Keywords: T7 RNA پلیمراز; dsDNA; double stranded DNA; T7 RNAP; T7 RNA polymerase; Protein tau; DNA replication; Transcription; Repression; PCR;
Enhancement of the in vitro transcription by T7 RNA polymerase of short DNA templates containing oxidative thymine lesions
Keywords: T7 RNA پلیمراز; Oxidative DNA damage; 5-(Hydroxymethyl)uracil; 5-Formyluracil; 5-Carboxyuracil; T7 RNA polymerase; In vitro transcription; Pausing signal; Dommages oxydatifs de l'ADN; 5-(Hydroxyméthyl)uracile; 5-Formyluracile; 5-Carboxyuracile; T7 ARN polymérase; Trans
In vitro coupled transcription splicing
Keywords: T7 RNA پلیمراز; Coupling; Cotranscriptional splicing; mRNA processing; RNA polymerase II; T7 RNA polymerase; Transcription;
Transcription arrest at DNA damage sites
Keywords: T7 RNA پلیمراز; T7 RNAP; T7 RNA polymerase; RNAPII; RNA polymerase II; GTFs; general transcription initiation factors; CPD; cyclobutane pyrimidine dimer; (6-4)PP; (6-4) photoproduct; cisplatin; diamminedichloroplatinum(II); cis-1,2-d(GG); cis-[Pt-(NH3)2{d(GpG)-N7(1),N7(2