کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1373984 1500612 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Alteration of cell wall polysaccharides through transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Alteration of cell wall polysaccharides through transgenic expression of UDP-Glc 4-epimerase-encoding genes in potato tubers
چکیده انگلیسی


• Both UGE transgenes were expected to modify cell wall galactose levels.
• UGE 45-1 transgenic potato had elongated pectin galactan side chains.
• Part of the pectin populations from UGE 51-16 potato had short side chains.
• Pectin of UGE transgenic lines had low acetylation and high methyl-esterification.
• Ratio of xyloglucan building blocks differed for UGE transgenic lines and wild-type.

Uridine diphosphate (UDP)-glucose 4-epimerase (UGE) catalyzes the conversion of UDP-glucose to UDP-galactose. Cell wall materials from the cv. Kardal (wild-type, background) and two UGE transgenic lines (UGE 45-1 and UGE 51-16) were isolated and fractionated. The galactose (Gal) content (mg/100 g tuber) from UGE 45-1 transgenic line was 38% higher than that of wild-type, and resulted in longer pectin side chains. The Gal content present in UGE 51-16 was 17% lower than that of wild-type, although most pectin populations maintained the same level of Gal. Both UGE transgenic lines showed unexpectedly a decrease in acetylation and an increase in methyl-esterification of pectin. Both UGE transgenic lines showed similar proportions of homogalacturonan and rhamnogalacturonan I within pectin backbone as the wild-type, except for the calcium-bound pectin fraction exhibiting relatively less rhamnogalacturonan I. Next to pectin modification, xyloglucan populations from both transgenic lines were altered resulting in different XSGG and XXGG proportion in comparison to wild-type.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 146, 1 August 2016, Pages 337–344
نویسندگان
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