کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1374755 | 981924 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Carbonic anhydrase inhibitors. Inhibition of the β-class enzyme from the yeast Saccharomyces cerevisiae with anions
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The protein encoded by the Nce103 gene of Saccharomyces cerevisiae, a β-carbonic anhydrase (CA, EC 4.2.1.1) designated as scCA, has been cloned, purified, characterized kinetically, and investigated for its inhibition with a series simple, inorganic anions such as halogenides, pseudohalogenides, bicarbonate, carbonate, nitrate, nitrite, hydrogen sulfide, bisulfite, perchlorate, sulfate, and some of its isosteric species. The enzyme showed high CO2 hydrase activity, with a kcat of 9.4 Ã 105 sâ1 and kcat/Km of 9.8 Ã 107 Mâ1 sâ1. scCA was weakly inhibited by metal poisons (cyanide, azide, cyanate, thiocyanate, KIs of 16.8-55.6 mM) and strongly inhibited by bromide, iodide, and sulfamide (KIs of 8.7-10.8 μM). The other investigated anions showed inhibition constants in the low millimolar range.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 24, 15 December 2008, Pages 6327-6331
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 24, 15 December 2008, Pages 6327-6331
نویسندگان
Semra Isik, Feray Kockar, Oktay Arslan, Ozen Ozensoy Guler, Alessio Innocenti, Claudiu T. Supuran,