Selective fluorescence quenching of the 8-oxoG-clamp by 8-oxodeoxyguanosine in ODN

The 8-oxoG-clamp, a specific fluorescent probe for 8-oxo-deoxyguanosine (8-oxo-dG), was incorporated into the oligodeoxynucleotide (ODN) within or at the 3′-end of the purine and the pyrimidine sequences. Based on the UV-melting temperature, the 8-oxoG-clamp showed slightly lower stabilizing effects on the duplexes containing 8-oxo-dG at the complementary site than that with dG. On the other hand, 8-oxo-dG in DNA was selectively detected by fluorescence quenching of the 8-oxoG-clamp.

The 8-oxoG-clamp, a specific fluorescence probe for 2′-deoxy-8-oxoguanosine (8-oxo-dG) in solutions, was incorporated into the ODN, which was shown to be a selective fluorescence probe for 8-oxo-dG in DNA.Figure optionsDownload as PowerPoint slide