کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1376089 1500658 2014 6 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
A cellulosic responsive “living” membrane
ترجمه فارسی عنوان
یک سلولوزی پاسخی به زندگی است غشاء
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کلمات کلیدی
غشای زنده، سلولز باکتریایی، همکاری تقویت کننده ژن، بازخورد مثبت
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی


• Demonstrated the compatibility of G. xylinus and E. coli for co-cultivation.
• Number of entrapped Escherichia coli correlated to inducible signal achieved.
• Validated a positive-feedback genetic amplifier loop.
• Demonstrated signal detection by eye.

Bacterial cellulose has been demonstrated to be a remarkably versatile biomaterial and widely used in biomedical applications due to its unique physical properties. Here we reported for the first time a “living membrane” system based on recombinant Escherichia coli bacterial strains entrapped in cellulosic membranes produced by Gluconacetobacter xylinus. Biologically driven detection and identification of a range of target molecules presents unique challenges, and requires that detection methods are developed to be rapid, specific and sensitive. The compatibility of G. xylinus and recombinant E. coli strains was first investigated for co-cultivation, and the relationship between the number of entrapped E. coli and the level of inducible signal achieved was further explored by fluorescent signal observation in confocal microscopy. Finally to amplify the response to inducers for maximum fluorescent signal, a positive-feedback genetic amplifier was designed within recombinant E. coli strain entrapped in the living cellulosic membrane system, allowing for the detection mechanism to be extremely sensitive and resulting in a significant fluorescent signal from a single receptor binding event. The living membrane system proposed here will create devices of greater complexity in function for applications in biological and chemical detection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 100, 16 January 2014, Pages 40–45
نویسندگان
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