کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1376511 981959 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selective photolabeling of Lck kinase in complex proteome
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Selective photolabeling of Lck kinase in complex proteome
چکیده انگلیسی

A molecular probe that selectively tags Lck, a Src-family kinase, was developed. This probe was one of many compounds originally designed to target the active site of tyrosine kinases in general. To our surprise, however, the probe almost exclusively labeled Lck even in a lysate of Jurkat cells. This finding led us to further characterize this probe-Lck complex by a series of photolabeling and mass spectrometric analyses. The probe-binding site on Lck was located within the well-conserved region of Src-family kinases, as we originally expected. However, the unexpected selectivity of this probe toward Lck suggests that subtle factors, which are difficult to predict based on static crystal structures, play important roles in probe recognition.

A simple molecular probe containing adenine and benzophenone was found to tag Lck in a highly selective manner.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 16, Issue 21, 1 November 2006, Pages 5625–5628
نویسندگان
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