کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1377635 | 981984 | 2006 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Non-covalent modification of the heme-pocket of apomyoglobin by a 1,10-phenanthroline derivative Non-covalent modification of the heme-pocket of apomyoglobin by a 1,10-phenanthroline derivative](/preview/png/1377635.png)
To expand the repertoire of artificial enzymes that are constructed by replacing the natural prosthetic group of hemoproteins with non-natural cofactors, we examined incorporation of a non-porphyrinic ligand (1) into the heme-pocket of apomyoglobin in a non-covalent fashion. Ligand 1 is a highly conjugated 1,10-phenanthroline derivative, which shares some structural features with protoporphyrin IX; for example, molecular size and arrangement of hydrophobic and anionic parts. Addition of apomyoglobin to a solution of 1 induces clear changes in the absorption spectrum of 1, suggesting one-to-one incorporation of 1 into the heme cavity of apomyoglobin with an affinity of 6.3 × 106 M−1. We found that the hydrolytic activity of apomyoglobin toward p-nitrophenyl hexanoate was greatly suppressed because of the incorporation of 1 into the heme-pocket.
To expand the repertoire of artificial enzymes constructed by replacement of prosthetic groups of hemoproteins, we examined incorporation of a non-porphyrinic ligand, a water-soluble 1,10-phenanthroline derivative (1), into the heme-pocket of apomyoglobin. Strong incorporation of 1 into the heme cavity of apomyoglobin greatly suppresses the hydrolytic activity of apomyoglobin toward p-nitrophenyl hexanoate.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 16, Issue 2, 15 January 2006, Pages 248–251