کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1378888 | 982012 | 2006 | 5 صفحه PDF | دانلود رایگان |
Two ligand–intercalator–peptide nucleic acid conjugates (L-NADI-PNAs) have been synthesized. Affinity of these conjugates to their complementary DNAs was found to be affected by Zn2+. The magnitude of this effect could be controlled by a variation of the ligand. Upon binding Zn2+ the L-NADI-PNAs form positively charged ZnL complexes, which interact with the negatively charged DNA backbone. This electrostatic interaction stabilizes PNA/DNA duplexes. It has been found that Zn2+ dependent stabilization takes place only if the ZnL complex has a higher total positive charge than the ligand. Linear correlation has been observed between Zn2+ induced stabilization of PNA/DNA duplexes and difference of charges of the ZnL complex and the ligand.
It has been found that affinity of a ligand–intercalator–peptide nucleic acid conjugate to its complementary DNA is affected by Zn2+ due to electrostatic interaction between the positively charged Zn(ligand) complex and the phosphodiester backbone of the DNA.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 16, Issue 10, 15 May 2006, Pages 2781–2785