Structural of a glucomannan from Lupinus varius seed

Polysaccharides extracted from seeds of Lupinus varius with hot ethanol 85% (polysaccharide FI) and 0.1 M sodium phosphate buffer, pH 7.5 (polysaccharide FII) were fractionated and purified by ion-exchange and gel-filtration chromatography. According to methylation and hydrolysis analysis, the main chains of FI and FII consisted of (1 → 4)-linked glucomannan; only traces of branched sugar residues were detected. This is the first report on the isolation of glucomannan from L. varius seeds.