Expanded acceptor substrates flexibility study of flavonol 7-O-rhamnosyltransferase, AtUGT89C1 from Arabidopsis thaliana

• We explore the expanded acceptor substrate flexibility of AtUGT89C1.
• We examine the catalytic potential of AtUGT89C1 using E. coli indigenous sugar.
• Library of flavonoids 7-O-rhamnoside could be produced using AtUGT89C1.

Acceptor substrates flexibility of previously characterized flavonol 7-O-rhamnosyltransferase (AtUGT89C1) from Arabidopsis thaliana was explored with an endogenous nucleotide diphosphate sugar and five different classes of flavonoids (flavonols, flavones, flavanones, chalcone and stilbenes) through a biotransformation approach. In contrast to the previous reports, this study highlights the expanded acceptor substrate promiscuity of AtUGT89C1 for the regiospecific glycosylation of diverse class of flavonoids at 7-hydroxyl position using microbial thymidine diphosphate (TDP)-l-rhamnose as sugar donor instead of uridine diphosphate-l-rhamnose. We examine the biocatalytic potential of AtUGT89C1 using endogenous sugar (TDP-l-rhamnose) from E. coli to generate a library of flavonoid 7-O-rhamnosides.

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