Lipopolysaccharide structure of Helicobacter pylori serogroup O:3

• Helicobacter pylori is the most common human pathogen.
• Lipopolysaccharide of H. pylori is its virulence factor and can be used for vaccine preparation.
• Previously published structure contained incorrect details.
• New structure is presented, based on the use of modern methods.

In this study, we describe a re-investigation of the lipopolysaccharide structure of Helicobacter pylori serogroup O:3. Application of NMR and MS approaches to the analysis of oligosaccharides obtained through degradation of LPS from H. pylori serogroup O:3 by various methods confirmed that its general architecture was identical to that of LPS from H. pylori strains 26695 and SS1 and followed a sequential linear assembly of the α-1,6-glucan, dd-heptan, and O-chain components. Additionally, MALDI-MS analysis demonstrated that a significant proportion of H. pylori serogroup O:3 LPS was terminated with α-1,6-glucan and was not further substituted by dd-heptan and the O-chain polysaccharide.

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