کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1384071 | 1500652 | 2014 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Structure and properties of a (1→3)-β-d-glucan from ultrasound-degraded exopolysaccharides of a medicinal fungus Structure and properties of a (1→3)-β-d-glucan from ultrasound-degraded exopolysaccharides of a medicinal fungus](/preview/png/1384071.png)
• Exopolysaccaride (EPS1) was isolated from Cs-HK1 mycelial fermentation broth.
• Ultrasonic degradation of EPS1 yielded EPS1U with higher water solubility.
• Purified EPS1U was elucidated as a branched (1→3)-β-d-glucan about 730 kDa.
• EPS1U showed high moisture absorption capacity similar to chitosan.
A high molecular weight (MW) exopolysaccaride (EPS) fraction EPS1 was isolated from the fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1 and partially degraded by high-intensity ultrasound (US) into a lower MW fraction EPS1U. EPS1U exhibited a single, symmetric peak on size exclusion chromatography with an average MW of 730 kDa by light scattering analysis. It had a much lower intrinsic viscosity (1.7 versus 15.6 dL/g) but a much higher solubility in water (77.5 versus 5.1 g/L) than EPS1. Based on methylation analysis and NMR spectrometry, the structure of EPS1U was deduced as a (1→3)-β-d-glucan with glucose side chains attached to O-6 position at the branching points. EPS1U showed a high moisture absorption capability comparable to chitosan and urea, suggesting its potential as a moisturizing agent for food and cosmeceutical application. This is the first report on a high MW (1→3)-β-d-glucan isolated from EPS produced by Cordyceps sinensis.
Journal: Carbohydrate Polymers - Volume 106, 15 June 2014, Pages 270–275