کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1384080 | 1500652 | 2014 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Purification, characterization and immunomodulating activity of a polysaccharide from flowers of Abelmoschus esculentus Purification, characterization and immunomodulating activity of a polysaccharide from flowers of Abelmoschus esculentus](/preview/png/1384080.png)
• A water-soluble polysaccharide OFPS11 was purified and preliminary characterized.
• OFPS11 could up-regulate protein and gene expression of NO and cytokines.
• NF-κB-mediated up-regulation of NO and TNF-α was observed.
• OFPS11 could be explored as a potential antitumor immunomodulatory agent.
A water-soluble polysaccharide (OFPS11) was obtained from okra (Abelmoschus esculentus) flowers using aqueous extraction and purification with DEAE-52 cellulose and Sephacryl™ S-500 column. Its preliminary characterization and immunomodulating activity were investigated. Results showed that OFPS11 is mainly composed of galactose and rhamnose in a molar ratio of 2.23:1 with molecular mass of 1700 kDa. RAW264.7 cells pretreated with OFPS11 significantly inhibited the proliferation of HepG-2 cells. Additionally, OFPS11 enhanced the phagocytic ability and induced the elevation of NO production, TNF-α and IL-1β secretion of RAW264.7 cells. Furthermore, OFPS11 promoted both the expression of iNOS protein and of iNOS and TNF-α mRNA. OFPS11 can strongly increase NF-κB levels in nucleuses, which is an important transcription factor that can modulate expressions of iNOS, NO and TNF-α. These outcomes support that OFPS11 exerts its antitumor activity by probably stimulating macrophage activities through nuclear NF-κB pathway.
Journal: Carbohydrate Polymers - Volume 106, 15 June 2014, Pages 335–342