Wide sugar substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4

Galactokinases (GALK) have attracted significant research attention for their potential application in the enzymatic synthesis of unique sugar phosphates. The galactokinase (GalKSpe4) cloned from Streptococcus pneumoniae TIGR4 had a temperature optimum of 45 °C, and a pH optimum of 8.0. The substrate specificity and kinetics studies revealed that GalKSpe4 had moderate activity toward glucose, in contrast with very low or no activity observed in other previously reported GALKs. Most interestingly, GalKSpe4 exhibited activity for GalNAc, which had never been recorded in other GALKs found by now. This is the first time to report that bacterial GALK can recognize GalNAc.

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► Galactokinase from Streptococcus pneumoniae TIGR4 has wide sugar substrate specificity.
► GalKSpe4 can recognize glucose as substrate.
► Most interestingly, GalKSpe4 exhibited activity for GalNAc.
► This is the first time to report that GALK in bacteria can recognize GalNAc.